The osmotic rupture hypothesis of intracellular freezing injury

Osmotic shrinkage as a factor in freezing injury in plant tissue cultures.

Haplopappus gracilis and Acer saccharum tissue culture cells are extremely sensitive to freezing injury, and exhibit a decrease in survival from 98% at -1 C to 4% at -3 C (Haplopappus) and 92% at -3 C to 13% at -5 C (Acer) when suspended in distilled H(2)O, seeded at -1 C, and then cooled by 0.1 C/minute. Similar results are obtained when cells are suspended in growth medium. The extent of shri...

Matrix Metalloproteinase Hypothesis of Plaque Rupture

Arterial thrombosis is generally recognized as the proximate event responsible for most acute ischemic syndromes resulting from atherosclerotic vascular disease.1,2 The majority of such thrombi (60% to 80%) occur at sites of fissure or rupture of a thinned fibrous cap overlying a lipid-rich atherosclerotic lesion with intimal and adventitial inflammation, and the remainder occur over areas of s...

Mechanism of Hyperosmotic and Extracellular Freezing Injury

The vome of isolated protoplasts of rye (Secale cereale L. cv Puma) in a suspending solution at constant concentration is shown to be neglgbly changed by tensions in the plasma membrane which approach that tension necessary to lyse them. This aflows a detailed investigation of the plasma membrane stress-strain relation by micropipette aspiration. Over periods less than a second, the membrane be...

Freezing of Gait Following Hypoxic Brain Injury

Corresponding author: Yong Wook Kim Department and Research Institute of Rehabilitation Medicine, Yonsei University College of Medicine, 250 Seongsanno, Seodaemun-gu, Seoul 120-752, Korea Tel: +82-2-2228-3716, Fax: +82-2-363-2795, E-mail: [email protected] Copyright © 2011 by Korean Academy of Rehabilitation Medicine J Korean Acad Rehab Med 2011; 35: 153-157 Case Report Freezing of Gait Following ...

Clinical Aspects of Freezing Cold Injury